| Solution Information | help | |
| Enzyme: | Tyrosine-protein kinase JAK2 | |
| inhibitor: | BDBM47478 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine whether compounds identified as active in a previous set of experiments entitled, "Primary cell-based high throughput screening assay to identify inhibitors of the mutant JAK2V617F" (PubChem AID 1446), and that confirmed activity in a set of experiments entitled, "Confirmation cell-based high throughput screening assay for inhibitors of the mutant JAK2V617F" (AID 1521), were nonselective due to inhibition of parental Ba/F3 cell line cytotoxicity. This assay also serves as a counterscreen for a set of assays entitled, "Luminescent dose response cell-based high throughput screening assay for inhibitors of the Janus kinase 2 mutant JAK2V617F" (PubChem AID 1691). In this assay, murine pro-B Ba/F3 cell viability is measured using the CellTiter-Glo luminescent reagent, which contains luciferase to catalyze the oxidation of beetle luciferin to oxyluciferin and light in the presence of ATP. As designed, compounds that inhibit cell via | |
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